The rate expression is where is the maximal reaction rate, is the Michaelis Menten constant and is the substrate concentration. The Lineweaver Burke plot can be used to determine both and - A plot of versus , will have a slope that is equal to and a y-intercept of

Consider this dataset which is the data from problem set 1, problem 14, Fall 2006. Here is how you can generate the plots using gnuplot

I then fit the two data sets to a straight line and plotted them on the same graph (I have not been able to include the gnuplot file here, but you can see the resulting image)

(The gnuplot command are listed here ... ) (do you know that you can upload your own file and generate plots like this if you want?)

The file used is set1prob14.dat (column 1 has the substrate concentration, column two for enzyme without inhibitor and column three with the inhibitor)

• f(x)=m1*x+b1
• g(x)=m2*x+b2
• fit f(x) "set1prob14.dat" using (1.0/$1):(1.0/$2) via m1,b1
• fit g(x) "set1prob14.dat" using (1.0/$1):(1.0/$3) via m2,b2
• set terminal png
• set output 'set1prob14.png'
• plot 'set1prob14.dat' using (1.0/$1):(1.0/$2), 'set1prob14.dat' using (1.0/$1):(1.0/$3), f(x), g(x)

Here is what you get after fitting - look at the difference in slopes, similar intercept

-- KrishnanChittur - 06 Sep 2006